MILTI-PURPOSE OPTICAL SPECTROMETERS FOR MEDICAL AND BILOLOGICAL STUDIES

E-mail: koker@kapella.gpi.ru

The family of all-in-one remote probe fluorescence spectrometers with built-in excitation lasers have been developed and are manufactured in General Physics Institute and Cluster Ltd. The spectrometers are applied for remote optical measurements from a surfaces and volume of different objects including reflectance and fluorescence spectra of biological objects.

The optical spectrometers are the unique devices which incorporate a grating polychromator with a multichannel photodetector together with up to three excitation lasers (FS003A - fluorescence spectrometer). The polychromator and the lasers are integrated into a CD-ROM-size module which is installed directly into a PC. The operating range of a spectrometer is 400-1000 nm. The choice of a laser source in 400-700 nm spectral range depends on the aim of the study. For remote fluorescence measurements the devices are equipped with the fiber optical probes having a length of up to 3 m and the outer diameter in 1.2-3 mm range. The probes are used for simultaneous excitation laser light delivery to an object, and for collection of fluorescence and reflected light from it. Spectral resolution of a spectrometer with fiber-optical probe is less then 3 nm. In the surface measurements, the spatial resolution can reach 1 mm. The modifications of the fiber-optical probes were developed for different applications which allow using the device in operation room, laboratories, and outdoor conditions including underwater measurements.

The spectral fluorescence analysis system Spectre-Cluster was developed on the base of this optical spectrometer jointly with P.A. Herzten Moscow Research Oncological Institute. The System is applied for fluorescence diagnostics of cancer and allows detecting small size malignant lesions of skin and hollow organ mucosa. The System was tested during the clinical trials of the all photosensitizers approved for photodynamic therapy of cancer in Russia (Photogem, Alasense, Photosense, Radachlorin, Photoditazin, Photolon). The long-term cooperation between our Institutes resulted in the development of the methods of local fluorescence spectroscopy (LFS) under multiple wavelength excitations in the different spectral ranges. The basic methodological approaches and indications for in vivo LFS applications in clinical oncology were also developed.

Except the fluorescence detection of cancer, the remote optical spectrometers have been effective in other applications including epifluorescence control in cosmetology, express diagnosis of virus contamination in leaves and some others.

 



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